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Abstract:
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The purposes of this study were to investigate the antibacterial activity of fruit extract of Carissa carandas
L. and to analyze alcohol-based-hand sanitizers mixed with C. carandas L. fruit extract. With regard to research
methodology, ethanol was used to extract C. carandas L. fruit. The samples were evaluated against 4 strains of
human pathogenic bacteria: Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella
typhimurium by broth dilution assay. The finding are that the extract of C. carandas L. showed 10 mg/mL
against L. monocytogenes, E. coli, S. typhimurium and 7.5 mg/mL minimal inhibitory concentration (MIC)
values for S. aureus. Minimal bactericidal concentration (MBC) of the crude extract was 30 mg/mL against S.
aureus, L. monocytogenes, E. coli and S. typhimurium. The MIC of the alcohol-based hand sanitizer mixed with
the C. carandas L. fruit extract was 5 mg/mL against S. aureus, L. monocytogenes, S. typhimurium and 10
mg/ml of E. coli. The MBC of the alcohol-based hand sanitizer mixed with C. carandas L. fruit extract was 10
mg/mL against S. aureus, L. monocytogenes, S. typhimurium and 20 mg/mL against E. coli. The hand sanitizing
product tested in this study is suitable for disease prevention. |