Antibacterial Activity of Alcohol-Based-Hand Sanitizer Mixed with the Carissa carandas L. Fruit Extract

Abstract

The purposes of this study were to investigate the antibacterial activity of fruit extract of Carissa carandas L. and to analyze alcohol-based-hand sanitizers mixed with C. carandas L. fruit extract. With regard to research methodology, ethanol was used to extract C. carandas L. fruit. The samples were evaluated against 4 strains of human pathogenic bacteria: Staphylococcus aureus, Listeria monocytogenes, Escherichia coli and Salmonella typhimurium by broth dilution assay. The finding are that the extract of C. carandas L. showed 10 mg/mL against L. monocytogenes, E. coli, S. typhimurium and 7.5 mg/mL minimal inhibitory concentration (MIC) values for S. aureus. Minimal bactericidal concentration (MBC) of the crude extract was 30 mg/mL against S. aureus, L. monocytogenes, E. coli and S. typhimurium. The MIC of the alcohol-based hand sanitizer mixed with the C. carandas L. fruit extract was 5 mg/mL against S. aureus, L. monocytogenes, S. typhimurium and 10 mg/ml of E. coli. The MBC of the alcohol-based hand sanitizer mixed with C. carandas L. fruit extract was 10 mg/mL against S. aureus, L. monocytogenes, S. typhimurium and 20 mg/mL against E. coli. The hand sanitizing product tested in this study is suitable for disease prevention.